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If you need more advanced functionality than mpsutil provides, LSI provides their native tools sas2ircu and sas3ircu for FreeBSD. On my system, this command produces a bright red LED lit for that slot, physically highlighting the correct drive to replace. So, to activate the LED for the first disk displayed above, we first need to determine the enclosure handle number (0001), and then the slot number of the disk (03). This partitions each disk and labels the ZFS partition with the enclosure, slot, and serial number of the corresponding disk. As with a number of tools in FreeBSD, sesutil supports outputting JSON via the libxo library.
SATA disks plugged directly into the motherboard use an interface called AHCI which does not provide much in the way of advanced management features. For smaller numbers of drives, and for most home systems, the most common way the disks are attached is to the SATA controllers built into the motherboard. Non-Volatile Memory Express (NVMe) is a newer storage interface that is becoming very popular for flash storage devices. Just download the executable file on both devices and run it to open the tool. At a glance, changing idle3 and EPC settings seems to have done the job nicely; here is the same graph of head park rates per disk as before, but on a smaller timescale that makes individual head parks visible. Seagate provide a “Seachest” collection of tools for manipulating their drives, but rather more usefully to users of non-Windows operating systems like Linux they also offer an open-source openSeaChest.

• It is very popular among professionals who provide technical support.
• 1 SSD to boot and 1 HDD to store data.
• The map command displays all of the SES devices and each element (this is the nomenclature in SES) connected to them.
• For ZFS users, automating fault responses with tools like ZED (ZFS Event Daemon) can simplify disk replacement and minimize downtime.
• Each SAS Expander will present as a new /dev/ses# device, so your system may have more than one.
• Many backplanes include support for SCSI Enclosure Services (SES).

Monitoring (and preventing) excessive hard drive head parking on Linux

This will activate the fault LED for element 9 (Slot 08) on the first SES device. You can avoid any uncertainty by enabling the “locate” or “fault” LED for the drive you mean to replace. This example creates a new GPT partition scheme on da36, creates a 4 GiB swap partition aligned to 1 MiB boundaries, and then adds a ZFS partition with the label e3s01-ZGY0XH87 using the remainder of the space on the disk.

• But, if the number of ports on the motherboard is sufficient to your needs, this is the easiest way to connect the drives to the system.
• Other interfaces for remote storage include iSCSI, Fiber-Channel, Infiniband, RoCE, and others, but those specialized solutions are beyond the scope of this article.
• (The properties like ID_SERIAL_SHORT can be queried on a running system using udevadm info, such as udevadm info /dev/sdd to get the properties of the disk currently assigned ID sdd.)
• ZFS and Btrfs both aim to modernize storage by combining filesystems and volume management, but…
• The first step is to map out the relationship between the physical chassis where the disks reside, and the logical devices enumerated by the operating system.

While I have been aware of this in my home server as well, it is easy to forget to ensure that disks are not silently killing themselves by cycling the heads. With modern, especially Enterprise grade hard drives being able to have hundreds of thousands of head park operations in their service life, is this really an isssue? With the tools presented here, the reader is well armed to react to failed disks and ensure that the wrong disk isn’t accidentally pulled. However, if a disk has died entirely, or a slot is empty, it might not have a device name. Sesutil can also be used to locate the disk in the physical array.While the SES data tells us that there is an 8 TB disk in Slot 06, it does not tell us which slot in the chassis corresponds to 06. Looking at a few items from the output, we can see the device names (/dev/da0 and /dev/da7 respectively) of the disks in Slot00 and Slot07.

Western Digital idle3

Direct Attached deployments require a bit more hardware and cabling. The NVMe interface is also extensible to allow operating over the network (where it is known as NVMe Over Fabric or NVMe-oF). NVMe on the other hand, supports multiple queues (often 64 queues, but the official specification allows for up to 65,536 queues) allowing for many commands to be run concurrently. While both SATA and SAS allow multiple commands to be issued at once to the device, these commands cannot actually be executed concurrently—instead, they are queued for sequential operation.
Most Seagate disks have configurable Extended Power Conditions (EPC) settings that include timers for how long the disk needs to stay idle before entering various low-power modes. Disk vendors typically provide their own vendor-specific ways to do persistent configuration of power management settings, so it’s worth trying to use those instead so the desired configuration doesn’t depend on the host system applying it, instead being configured in the drive (but in some cases it might be desirable to have the host configure that!). To prevent parking the heads at all a value greater than 128 may do the job (254 is a common choice, as the highest-power setting available), but it’s possible that some disks won’t behave this way because the ATA specification refers only to spinning down the disk and does not specify anything about parking heads. Typical SAS connectors support up to 4 drives per “lane”, but with an expander up to 255 devices are possible. An eight lane controller can only directly attach to 8 disks, requiring more controllers (consuming additional PCI-E slots) to connect more drives. This has long been the interface bus used by most home users to connect their hard drives, and is supported by nearly every motherboard.
The parking rate basically drops to zero at the time I updated the settings for the Seagate drives, and the Western Digital one hasn’t changed because it needs to be powered off to change that setting and I haven’t done so yet. The other slight annoyance when setting the idle3 timer on WD drives is that changes only take effect when the drive is powered on, usually meaning the host computer must be fully shut down and started back up for any changes to be seen- this makes experimentation to determine how raw timer values are interpreted a slower and more tedious process. Of particular note, WD Green drives ship configured to park reveryplay the heads after only 8 seconds of inactivity which could notionally wear out the disk in a matter of months if the heads are cycling more-or-less continuously! For drives made by Western Digital, the inactivity timer for parking the heads is called the idle3 timer.

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I will optimize settings later for the security/quietness tradeoff however, I’m very pleased with it for now. How can I set this value on the Truenas interface? Keeping it spinning but not accessing data is safer. I would still recommend against idling your drive as that reduces longevity. I also set the tunable vfs.zfs.txg.timeout to a somewhat large value so the regular syncs don’t happen every 5 seconds.
Once you’ve done so, you must test delivery to your “real” inbox—you don’t want to learn that delivery isn’t working after your storage has already become unavailable! If you’d feel safer with a team of experts monitoring your storage, consider a ZFS Support Subscription. If you rely on manually checking on your storage periodically, you will regret it. Another important aspect of managing your storage system is configuring notifications. Klara recommends embedding these details directly into the ZFS vdev properties of each disk—a feature Klara created, which will become generally available in the upcoming OpenZFS 2.2 release. In these configurations, your system may or may not support features like individual “locate” and “fault” LEDs.

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When it comes to long-term data storage, there are several strategies and media types that Redditors recommend. It refreshes the disks SMART information every 5 min. ZFS and Btrfs both aim to modernize storage by combining filesystems and volume management, but… Monitoring and maintaining your storage media is one of the most important parts of keeping your data safe.
I noticed that even when doing nothing, I hear the sound of drives working every few seconds. I gave up and just built a Windows Storage Space with tiering and the drives are now effectively silent. I guess it depends on the drives, but don’t think you’ll find any software solution. My Seagate Exos enterprise drives make almost 0 noise actually. The system is never idle really, it’s a server. What causes the constant load on the disk?

I agree to receive your newsletters and accept the data privacy statement. Ensure device health & easy replacements with these valuable tips. Discover strategies to manage disk arrays on FreeBSD and related platforms/operating systems. Simply installing the apps and choosing a pool for k3s and docker creates a dataset and logs. Your pool gets writes from somewhere and ZFS is writing those to disk every 5 seconds.

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I moved the system dataset to the boot pool. I don’t move any data, no apps are running, this is a vanilla Scale install so far, yet the HDD is in constant work. 1 SSD to boot and 1 HDD to store data. Agree, I have used SeaChest with good results for this same issue on scale plus drive cache. If you do it on a live pool, I’d back up your data first.
The settings you mentioned are already set this way. After you apply these settings the logs will be written to your SSD instead of being flushed to the disc array. Those are probably the system logs being flushed to disk every few seconds. I have moved the system data to my boot SSDs, don’t have any apps installed and don’t have any pool set for apps.
For ZFS users, automating fault responses with tools like ZED (ZFS Event Daemon) can simplify disk replacement and minimize downtime. Configuring your system to notify you when a disk has errors, or when the filesystem reports a degraded device, will ensure your system gets prompt attention when something goes wrong. Experienced enterprise storage managers also keep extensive notes including the model number, SKU and/or URL for reordering, purchase order information, warranty end date, warranty URL, and any other useful information about each drive. While the operating system typically provides device aliases based on the disk’s serial number, WWN, or some other static identifier, this does not provide all of the information you might want.
It is fairly well-known among techies that hard drives used in server-like workloads can suffer from poor configuration by default such that they frequently load and unload their heads, which can cause disks to fail much faster than they otherwise would. My Seagate Archive SMR disk (which began life as an external hard drive and was retired from that role when it became too small to hold as much as I wanted to back up to it) apparently doesn’t support reporting EPC settings (since asking for them says so), and initially didn’t accept new values for the idle timers either. The Prometheus Node Exporter is the canonical tool for capturing machine metrics like utilization and hardware information with Prometheus, but it alone does not support probing SMART data from storage drives. While SSDs don’t have any heads to park, most do report a media_wearout_indicator that represents the amount of data written to the device in relation to the amount that it’s specified to accept before the Flash storage medium wears out.
It’s hard to imagine why your drives are that loud! It’s a datacenter drive, very loud, so it’s still audible. For quietness, a noise reducing case, move it somewhere else, quieter drives, maybe SSD instead of hard drives, etc.
Sounds like the drives being woken for the ZIL to flush writes to the ZFS pool and then going back to idle/sleep every 5 seconds. Enable the checkmark for the Syslog and choose a pool that is not based on hard drives. I had this same problem, using HGST data center refurb drives.

When it comes to long-term data storage, there are several strategies and media types that Redditors recommend. It refreshes the disks SMART information every 5 min. ZFS and Btrfs both aim to modernize storage by combining filesystems and volume management, but… Monitoring and maintaining your storage media is one of the most important parts of keeping your data safe.

• Many backplanes include support for SCSI Enclosure Services (SES).
• 1 SSD to boot and 1 HDD to store data.
• Each SAS Expander will present as a new /dev/ses# device, so your system may have more than one.
• It is very popular among professionals who provide technical support.
• The map command displays all of the SES devices and each element (this is the nomenclature in SES) connected to them.
• If we wanted to allow the disk to still park its heads but at minimum frequency, setting the APM value to 7Fh (hdparm -B 127) seems to be the correct choice.

However, I noticed that my HDD’s heads park (particulary Seagate Exos) every 3 minutes. ZFS is widely trusted for large-scale storage, but production environments expose design mistakes,… When dealing with critical data, you only get one chance to do it right. The status field is a bitmask supporting a number of different options, but the main ones we care about are 1 (OK), and 2 (FAULTED). When combined with a JSON parser like jq, this can be used to automate tasks for each disk.
Sounds like the drives being woken for the ZIL to flush writes to the ZFS pool and then going back to idle/sleep every 5 seconds. Enable the checkmark for the Syslog and choose a pool that is not based on hard drives. I had this same problem, using HGST data center refurb drives.
I moved my Scale server into the next room, laundry room, just so it’s out of sight. Replacing the drive is financially out of the question. I’m looking for a software solution, if possible, to make the HDD idling for most of the time when there is no load. Yeah, it’s not helping, thanks. Although it’s empty, so this is probably not the source of the constant HDD noise.
The settings you mentioned are already set this way. After you apply these settings the logs will be written to your SSD instead of being flushed to the disc array. Those are probably the system logs being flushed to disk every few seconds. I have moved the system data to my boot SSDs, don’t have any apps installed and don’t have any pool set for apps.

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• This partitions each disk and labels the ZFS partition with the enclosure, slot, and serial number of the corresponding disk.
• The timer values specified are in milliseconds, so this example will park the disk heads after 30 minutes of inactivity.
• You should also configure smartd to monitor your disks and send you alerts, which may give you advanced notice when a drive is starting to fail.
• I guess it depends on the drives, but don’t think you’ll find any software solution.
• At a glance, changing idle3 and EPC settings seems to have done the job nicely; here is the same graph of head park rates per disk as before, but on a smaller timescale that makes individual head parks visible.
• Serial Attached SCSI (SAS) is the most common interface for enterprise storage, first appearing in 2004.
• For example, a device may implement one power management method from 80h to A0h and a higherperformance, higher power consumption method from level A1h to FEh.

I noticed that even when doing nothing, I hear the sound of drives working every few seconds. I gave up and just built a Windows Storage Space with tiering and the drives are now effectively silent. I guess it depends on the drives, but don’t think you’ll find any software solution. My Seagate Exos enterprise drives make almost 0 noise actually. The system is never idle really, it’s a server. What causes the constant load on the disk?
My question is – is there a way to tell if a certain disk suffers from the issue prior to purchasing? For the system I’m monitoring here, the SSD that it boots from has a wearout indicator sitting on 95 of 100 (only 5% of the rated life consumed), visibly unchanged for a long time so it’s not very interesting as an example. (The properties like ID_SERIAL_SHORT can be queried on a running system using udevadm info, such as udevadm info /dev/sdd to get the properties of the disk currently assigned ID sdd.) Somewhat more useful for monitoring is the smartmon_load_cycle_count_raw_value, which provides the actual number of load cycles that have been done. Secondly what are your disk monitoring refresh intervals and what do you use on your system to monitor SMART disk health?
The timer values specified are in milliseconds, so this example will park the disk heads after 30 minutes of inactivity. If we wanted to allow the disk to still park its heads but at minimum frequency, setting the APM value to 7Fh (hdparm -B 127) seems to be the correct choice. Of the three disks that I decided need some attention, I have one Western Digital disk and two Seagate ones.

Labeling with GEOM Multipath

The APM specification dating from 1992 includes some controls for hard drives, allowing a host system to specify the desired performance level of a disk and whether standby is permitted by sending commands to a disk. In addition to the above query types, SES also supports a number of commands, including activating the “locate” and “fault” LEDs if present, and the ability to individually power off drives. The first step is to map out the relationship between the physical chassis where the disks reside, and the logical devices enumerated by the operating system.

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I will optimize settings later for the security/quietness tradeoff however, I’m very pleased with it for now. How can I set this value on the Truenas interface? Keeping it spinning but not accessing data is safer. I would still recommend against idling your drive as that reduces longevity. I also set the tunable vfs.zfs.txg.timeout to a somewhat large value so the regular syncs don’t happen every 5 seconds.
Other interfaces for remote storage include iSCSI, Fiber-Channel, Infiniband, RoCE, and others, but those specialized solutions are beyond the scope of this article. Serial Attached SCSI (SAS) is the most common interface for enterprise storage, first appearing in 2004. Serial ATA (SATA) is the familiar interface used for non-enterprise storage, and is an extension of the original ATA interface dating from the 1980s. In this article we will discuss some strategies and tools to make managing disk arrays on FreeBSD (and related platforms like TrueNAS Core) much easier. It may be what you want is to enable HDD standby, which will “spin down” the drives when not in use
For ZFS users, automating fault responses with tools like ZED (ZFS Event Daemon) can simplify disk replacement and minimize downtime. Configuring your system to notify you when a disk has errors, or when the filesystem reports a degraded device, will ensure your system gets prompt attention when something goes wrong. Experienced enterprise storage managers also keep extensive notes including the model number, SKU and/or URL for reordering, purchase order information, warranty end date, warranty URL, and any other useful information about each drive. While the operating system typically provides device aliases based on the disk’s serial number, WWN, or some other static identifier, this does not provide all of the information you might want.
If your system has multipath SAS, each disk will be present more than once, and you should use the gmultipathcommand to deduplicate your disks and for labeling as well. FreeBSD supports a number of different ways to label the disk, depending on your use case. The map command displays all of the SES devices and each element (this is the nomenclature in SES) connected to them. Of course, all of this chassis management technology isn’t very effective without tools to make it usable. It also provides information about each slot in the enclosure (even if empty), including a flag to indicate if the device has recently been swapped.

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Once you’ve done so, you must test delivery to your “real” inbox—you don’t want to learn that delivery isn’t working after your storage has already become unavailable! If you’d feel safer with a team of experts monitoring your storage, consider a ZFS Support Subscription. If you rely on manually checking on your storage periodically, you will regret it. Another important aspect of managing your storage system is configuring notifications. Klara recommends embedding these details directly into the ZFS vdev properties of each disk—a feature Klara created, which will become generally available in the upcoming OpenZFS 2.2 release. In these configurations, your system may or may not support features like individual “locate” and “fault” LEDs.
While I have been aware of this in my home server as well, it is easy to forget to ensure that disks are not silently killing themselves by cycling the heads. With modern, especially Enterprise grade hard drives being able to have hundreds of thousands of head park operations in their service life, is this really an isssue? With the tools presented here, the reader is well armed to react to failed disks and ensure that the wrong disk isn’t accidentally pulled. However, if a disk has died entirely, or a slot is empty, it might not have a device name. Sesutil can also be used reveryplay to locate the disk in the physical array.While the SES data tells us that there is an 8 TB disk in Slot 06, it does not tell us which slot in the chassis corresponds to 06. Looking at a few items from the output, we can see the device names (/dev/da0 and /dev/da7 respectively) of the disks in Slot00 and Slot07.

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It is fairly well-known among techies that hard drives used in server-like workloads can suffer from poor configuration by default such that they frequently load and unload their heads, which can cause disks to fail much faster than they otherwise would. My Seagate Archive SMR disk (which began life as an external hard drive and was retired from that role when it became too small to hold as much as I wanted to back up to it) apparently doesn’t support reporting EPC settings (since asking for them says so), and initially didn’t accept new values for the idle timers either. The Prometheus Node Exporter is the canonical tool for capturing machine metrics like utilization and hardware information with Prometheus, but it alone does not support probing SMART data from storage drives. While SSDs don’t have any heads to park, most do report a media_wearout_indicator that represents the amount of data written to the device in relation to the amount that it’s specified to accept before the Flash storage medium wears out.

sesutil status

Using the no-op true command on other paths to that disk, will cause GEOM to re-”taste” the disk and see the label and automatically add the additional paths to the existing multipath. This will write a GEOM Multipath label to the last sector of the disk. Each SAS Expander will present as a new /dev/ses# device, so your system may have more than one.
Unnamed devices can be specified by their specific SES device and element number. This greatly reduces the chance of getting it wrong when you (or the datacenter technician) physically pulls the disk. You can also reboot, and GEOM will pick up the multipath when it first tastes the disks during boot.

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It is also worth mentioning that multiple components of the InsR pathway, including age-1, pdk-1, akt-2, and daf-16, are predicted to be targets of the let-7 family miRNAs. Our data provide the experimental evidence that two components of the InsR pathway are likely direct targets of miR-71 in its role in a specific physiological process, L1 diapause (see a model in Fig. S5). Components of the InsR pathway, including age-1, have recently been predicted to be targets of miR-71 in its role in aging (14).

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• To test whether the activity of the InsR pathway was down-regulated by miR-71, we first examined the endogenous expression of components of the InsR pathway in mir-71(lf).
• We recommend that incorporating trait-based recovery dynamics is essential for predicting ecosystem stability under compound climate extremes.
• DAF-16 (the FOXO homolog in C. elegans) has been shown to play an important role in cell cycle arrest and developmental progression partly by promoting cki-1 expression in some somatic cells during L1 arrest (2).
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The primers that were used to amplify the 3′UTR of candidate genes are available upon request. 3′UTRs of genes of interest were cloned into the modified pPD129.57 vector as described previously (18). The data for 3′UTR expression and for VPC timing were analyzed using χ2 test.
To determine viability, 20-μL aliquots (60–100 worms) were placed every 3 d onto two 6-cm nematode growth medium (NGM) plates seeded with OP50, and the numbers of L1 worms were recorded as number of plated worms (Np). A total of 16–24 h later, the density of newly hatched L1 worms was adjusted to three to five worms per microliter S-basal. The eggs were transferred to plates seeded with HB101 and bleached again 3 d later. Briefly, worms were well fed for at least two generations, and gravid adults were bleached with hypochlorite and sodium hydroxide. L1 starvation assay was adapted from a previously described protocol (3). Worms strains were grown and maintained at 20 °C as described (29).

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When late, first larval stage (L1) worms sense unfavorable conditions, they enter an alternative and long-lived larval stage called dauer larvae (or dauer diapause). The nematode Caenorhabditis elegans responds to starvation by entering developmental arrest at multiple stages of its life cycle (1). Extreme climate events such as droughts and heatwaves are intensifying under climate change, yet their combined effects on plant recovery remain unclear. These pages contain all relevant country-specific information, including the recovery and resilience plans, the Commission’s assessment of the plans as well as information on payments requested by the Member States and funds paid out by the Commission.
Furthermore, worms that are long-lived due to dietary restriction or decreased mitochondrial respiratory rates are short-lived during L1 diapause, suggesting that the mechanisms controlling L1 starvation survival are different at least in some aspects from those controlling aging (3). We provide evidence that miRNA miR-71 is not required for the animals’ entry into L1 diapause, but plays a critical role in long-term survival by repressing the expression of insulin receptor/PI3K pathway genes and genes acting downstream or in parallel to the pathway. Full device encrypted backups to iTunes will back up both the account listings and private key pairs, but can only be restored on the SAME phone that created the backup. If you enabled third-party account backup, you can recover your accounts on your iOS or Android device. If your organization hasn’t enabled self-service device management, contact your IT Help Desk or Duo service administrator for assistance reactivating the account.
The RRF funding amounts shown for measures are based on the initial cost estimates provided by the Member States included in the recovery and resilience plans and may as such differ from finalized Commission financial reports, which follow eventual project implementation. The funding amounts shown reflect the initial cost estimates included in the national recovery and resilience plans. Member States use the funds provided by the Recovery and Resilience Facility to implement ambitious reforms and investment to make their economies and societies more sustainable, resilient and prepared for the green and digital transitions. The Scoreboard gives an overview of progress in implementing the Facility and the national recovery and resilience plans.

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On the other hand, the role of a particular miRNA (miR-71) is executed by repressing the expression of many genes in multiple pathways. On one hand, we showed that deletions of a good number of miRNAs have varying impacts on the L1 diapause survival rate, although they may effect the rate through different mechanisms. Instead, many specific physiological functions, such as the starvation-induced stress response, are regulated by a miRNA-target network, often involving multiple miRNAs and a large number of their targets. We found that the known developmental timing genes, hbl-1, lin-42, and lit-1, were at the top of the list (TargetScan). To understand how miR-71 affects VPC division, we searched its predicted targets for potential genes involved in regulating developmental timing. These results indicate that miR-71 plays a significant role in larval development of animals recovering from L1 diapause and likely does so by regulating the expression of components of the insulin receptor/DAF-16 pathway, as well as factors acting downstream, or in parallel to, DAF-16.

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• (H) Fluorescence and DIC images showing that a lin-42 3′UTR reporter was repressed in mir-71(+) worms (2/2 transgenic lines) and prominently derepressed in mir-71(−) worms (2/2 transgenic lines).
• (H and I) Fluorescence images (H) and statistical data (I) showing that the M cell diveded in fed animals but remained undivided in 4-, 7-, or 11-d–starved L1 wild-type and mir-71(lf) worms.
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Whereas the vulva of wild-type worms developed into the pyramidal stage (81 of 82 worms), the P6.p of mir-71(n4115, lf) mutant worms divided only once (83 of 89 worms). The computation-based prediction that age-1 and pdk-1 are potential targets of miR-71 was also reported in a recent study focusing on miRNA functions in aging where the mRNA level of pdk-1 was shown to be up-regulated in mir-71 worms (14). (C) Fluorescence and differential interference contrast (DIC) images showing that the age-1 3′UTR reporter was repressed in mir-71(+) worms (3/4 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines). The transcript level of unc-31 was increased in mir-71(lf) worms, compared with that of wild-type controls that were normalized to the value of 1. MiR-71 represses the expression of age-1 and unc-31 through the actions on their 3′UTR, but miR-71 is not required for arresting M cell division during L1 diapause.

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• Individual GFP reporter constructs for candidate genes (4 ng/μL) and the mCherry internal control plasmid (4 ng/μL) were mixed with unc-119 rescuing plasmid (20 ng/μL) and pBluescript KS+ (72 ng/μL) and coinjected into unc-119(ed3) and mir-71(n4115); unc-119(ed3) worms following standard protocols (32).
• Elegans and Caenorhabditis briggsae, leading us to focus further analyses on these two genes.
• As pointed out above, multiple miRNAs in addition to miR-71 and the let-7 family miRNAs have roles in L1 diapause, and they may regulate the expression of many diverse targets that may include, but are not limited to, factors involved in UNC-31–InsR-signaling activities.

Here we show that compromising overall microRNA (miRNA) functions or mutating certain individual miRNAs impairs the long-term survival of nematodes during starvation-induced L1 diapause. Third-party accounts will also be restored if third-party backup was enabled on the old device. If you become locked out of those services and don’t have a backup of your accounts in Duo Mobile, you’ll need to contact the support team for that application (or perform the account recovery process for each of those third-party applications). However, whether an account can be restored depends upon Duo Restore being enabled by the administrator in the Duo Admin Panel or whether you’ve set a recovery password for reconnecting third-party accounts.
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{We further examined worms recovering from 4 d of L1 starvation and found that around 90% of the mir-71(lf) mutants displayed retarded vulval precursor cell (VPC) division, compared with less than 5% in wild type (Fig. 4A). We found that the 3′UTRs of several genes of the InsR pathway, including unc-31, age-1, pdk-1, akt-2, and sgk-1, contain predicted miR-71 targeting sites (as predicted by TargetScan and mirWIP). (H and I) Fluorescence images (H) and statistical data (I) showing that the M cell diveded in fed animals but remained undivided in 4-, 7-, or 11-d–starved L1 wild-type and mir-71(lf) worms. (E) Fluorescence and DIC images showing that the unc-31 3′UTR reporter was repressed in mir-71(+)worms (2/2 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines). We found that the poor survival rate of daf-16(mu86)(lf) was further decreased by mir-71(lf) (Fig. 2C), consistent with the notion that a portion of miR-71 activities regulate genes that act in parallel to UNC-31–mediated InsR/PI3K signaling for long-term survival during L1 diapause. Mutating miR-71 drastically reduces the survival rate of animals in L1 diapause, and the effect can be suppressed by mutations of insulin receptor pathway genes age-1 and unc-31.}

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This type of therapy focuses on the identification of gambling-related thought processes, mood and cognitive distortions that increase one’s vulnerability to out-of-control gambling. One form of counseling, cognitive behavioral therapy (CBT) has been shown to reduce symptoms and gambling-related urges. The VGS has proven validity and reliability in population studies as well as Adolescents and clinic gamblers.citation needed The DSM-IV diagnostic criteria presented as a checklist is an alternative to SOGS, it focuses on the psychological motivations underpinning problem gambling and was developed by the American Psychiatric Association. Life History Theory further explains why some individuals are more vulnerable to problem gambling, proposing that those with a “fast” strategy (marked by impulsiveness and short-term planning) may be especially drawn to high-risk, high-reward scenarios.

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“They should be aware of what their children are doing, and on the other hand, they should motivate pro-active gambling, healthy gambling”, commented Lizbeth García Quevedo. The 194-member meet added excessive gaming to a classified list of diseases as it revised its International Statistical Classification of Diseases and Related Health Problems (ICD-11).citation needed In its 72nd World Health Assembly held on Saturday, May 25, 2019, ‘gaming disorder’ was recognized as an official illness. through their reclassification of gambling as a disease, and therefore that it should be addressed adequately by the NHS.}

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